Interplay of selected efflux protein inhibitors on the antibacterial activity and pharmacokinetics of enrofloxacin in Chicken

Abstract

The present study was designed to determine the interplay of efflux protein inhibitors on the antibacterial activity and pharmacokinetics of enrofloxacin in chicken. Capsaicin and theobromine were used as efflux protein inhibitors. Birds weighing about 1.5 to 2.0 kg were allocated to 3 groups of six at random. Group I served as enrofloxacin control, whereas Group II and III birds were coad ministered with capsaicin and theobromine along with enrofloxacin. The plasma levels of enrofloxacin was determined by HPLC. The pharmacokinetic parameters were estimated by using Pksolver. Coadministration theobromine improved the bioavailability of enrofloxacin. The antibacterial activity was assessed by using microbroth dilution technique as per CLSI guidelines using MIC as determinant against ATCC strains of E. coli, S. aureus, K. pnemoniae, P. aureginosae. The results obtained in the study reveaed that combination of enrofloxacin with capsaicin and the obrominne enhanced the antibacterial activity of enrofloxacin by reducing the MIC values. The combination may be beneficial in reducing the development of resistance after conducting further studies.