Monitoring the degradation of total petroleum hydrocarbon in hydrocarbon impacted site using a consortium of hydrocarbon utilizing bacteria

Abstract

Bacteria (Hydrocarbon utilizing Bacteria) are ever present in natural history, and they make use of these blends as the origin of carbon and energy, however bacteria with such potential are frequently utilized for bioremediation of fossil fuel polluted earth, and have develop corrective measures against petroleum contaminants, by improving the bonding capacity of their cells changing their exterior mechanism and secreting surface active biomolecule materials to augment contact to objective hydrocarbon substrates. The essence of the study is to examine the degradation of total petroleum hydrocarbon in fossil oil polluted soil matrix using consortium of bacteria in three concentrations. (10%, 1.0% 0.1%. for 16 weeks). Consortium of bacteria used is Bacillus thuringiensis, Bacillus velezensis and Stenotrophomonas species. However result from the Gas chromatography flame induction detector (GC-FID) analysis showed the extent of degradation 10% (948.46±0.07 mg/kg and 2.97±0.01 mg/kg), 1.0% (110.07±0.10 mg/kg and 21.95±0.08 mg/kg) and 0.1% (99.99±0.01 mg/kg and 13.88±0.04), at the initial and final stage of exposure respectively. The study specifies that, augmenting biodegradation prospective via the application of bacterial consortia with several catabolic genes, is practical and possible approach for facilitating the elimination competence of petroleum hydrocarbons from contaminated environments.