Ageratum conyzoides L. Protects rat myocardium during isoproterenol-induced myocardial infarction in rats: A biochemical study

Abstract

This study aimed to evaluate the preventive effect of Ageratum conyzoides L. on cardiac Troponin T (cTnT), lactate dehydrogenase (LDH)-isoenzyme, cardiac marker enzymes, blood glucose, total proteins, albumin/globulin (A/G) ratio, serum uric acid, serum iron, and plasma iron binding capacity in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Rats treated with ISO(85 mg/kg, administered subcutaneously twice at an interval of 24 h for 2 days) showed a significant increase in the degrees of cardiac troponin T (cTnT), the intensity of the bands of LDH1 and LDH2, and the activities of cardiac diagnostic marker enzymes such as creatine kinase-MB (CK-MB), creatine kinase (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST) and alanine transaminase (ALT) in serum with a subsequent decrease in the activities of CK, LDH, AST and ALT in the heart. ISO-induced rats showed a significant increase in blood glucose, serum uric acid, and serum iron and a decrease in the levels of total proteins, A/G ratio, and iron binding capacity. Pre-treatment with A. conyzoides L. (100 and 200 mg/kg) daily for a period of 30 days significantly altered the levels of cTnT, the intensity of the bands of LDH1, and LDH2-isoenzyme and the activities of cardiac marker enzymes, and other biochemical parameters. Thus, A. conyzoides L. possesses a cardioprotective effect in ISO-induced oxidative stress in rats.