Ethanol intoxicated renal oxidative stress mitigated by poly-herbal formulation-Trasinareg; in murine model

Abstract

Oxidative stress has a critical role in the pathophysiology of several kidney diseases. Safe and symptomatic medication to prevent the kidney oxidative stress id highly recommended. In this study the aim and objective of this scientific study was to find out the probable protective effect of poly-herbal formulation (Trasina®) on serum and kidney antioxidant enzymes activities in ethanol intoxicated organ dysfunctions in mice model. Forty Swiss albino adult male mice were randomly divided into four groups; Group-1 served as control, Group-2 orally treated with ethanol (50% v/v), Group-3 pre-treated with herbal medicine (Trasina®) along with ethanol (50% v/v), and Group-4 only treated with poly-herbal formulation (Trasina®) without ethanol daily. Completion of six weeks treatment the animals were euthanized and kidneys were immediately removed and used fresh or kept frozen until analysis. Before sacrifice blood samples were taken to measure the antioxidant parameters i.e. super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione –S transferees (GST) from sera. Super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione–S transferees (GST) activities in serum and kidney were significantly reduced in the ethanol intoxicated mice than in the controls. Treatment with herbal medicine (Trasina®) upon ethanol intoxication significant elevated the all antioxidant activities serum and kidney. Results obtained from the present study clearly predict that treatment with poly-herbal formulation (Trasina®) might be a potent antioxidant that exerts beneficial effects on both super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione –S transferees (GST) activities in ethanol intoxicated mice and inhibit organ damage.