Effect of gentamicin on renal function, oxidative stress, and some markers of antioxidants in male rats

Abstract

Gentamicin overdose is a frequent cause of nephrotoxicity and possible death, so this study aimed to investigate the effect of gentamicin on serum renal enzyme, oxidative stress, and some markers of antioxidants in male rats. Methods: A total of 20 rats (3 to 6 months old) raised at high ambient temperature were divided into two equal groups, 10 rats each. The first control group was administered normal saline and the second group of rats was treated with gentamycin (80 mg/kg body weight by intraperitoneal injection) for 30 days. Blood samples were drawn to measure blood urea nitrogen (BUN) levels, serum creatinine, total protein, glutathione (GSH), Malondialdehyde (MDA), and catalase (CAT) levels, and activities were evaluated. Results: In the gentamicin-treated group, significant increases in blood urea nitrogen activities, serum creatinine, total protein activities, malondialdehyde (MDA) levels, and a significant decrease in catalase (CAT) and glutathione activities were determined compared to the control group. Conclusion: Administration of gentamycin produces notable biochemical changes in a dose-dependent manner associated with increased renal enzyme, markers of oxidative stress, and decreased antioxidant activity.