Hydrogen peroxide scavenging capacity and phytochemical analysis of Medicago sativa (Alfalfa) leaves

Abstract

This research work was carried out to evaluate the hydrogen peroxide scavenging capacity and phytochemical analysis of Medicago sativa leaves. The leaves were found to have high antioxidant activities. Medicago sativa extract caused inhibition of hydrogen peroxide which was concentration dependent. From the analysis, it was discovered that at every concentration, the methanol extract of the leaves showed better scavenging capacity than the standard used. The phytochemicals from the leaves of Medicago sativa (Linn.) were extracted with ethanol and subjected to GC/MS analysis. The chromatogram depicted five peaks indicating the presence of five compounds. 1,1-dimethoxy-2-methylpropane (59.781 %), 1,3-dimethyl Benzene (16.688 %), Ethanol, 2-(2-butoxyethoxy) acetate (2.788 %), N-(4-Methylbenzenesulfonyl)-2-methylazetidin-3-one (1.921 %). Brief analysis of the compounds reveals the rich pharmacological potential of this part of the plant and thus justify the use of the Leaves of this plant in the treatment of various diseases