Phytochemical, anti-sickling and polymerization inhibition studies of the crude methanol leaf recipe extracts of three medicinal plants

Abstract

This research focuses on the evaluation of herbal recipe made from Carica papaya linn, Psidum guajava linn. and Terminalia catappa linn which are used individually and as a recipe for the treatment of Sickled cell disease (SCD) in northern Nigeria. The research seek to validate the traditional application of the recipe in the management of sickle cell disease and to further identify its phytoconstituents (qualitatively), anti-sickling and polymerization inhibition potential in an in-vitro system. The results of the phyto-evaluation revealed the presence of alkaloids, cardiac glycosides, flavonoids, tannins, terpenoids, phenolics, and saponins, while the sickling inhibition studies showed a significantly higher inhibition level in dose dependent manner of up to 71.32% and 85.71%. The results for sickling reversal studies showed a similar trend with reversal levels of up to 92.03% and 94.51% and a polymerization inhibition effect of 79.84% to 99.18% respectively. Thus, the recipe formulated from the three commonly used plants may Served as an excellent candidate for the development of plant drug for the management of SCD, may Served as a prophylactic agent that will reduced the frequency of crisis in sickle cell patients.