Studies on Escherichia coli LPS and Neem oil intra-uterine therapy in infectious repeat breeder cows

Abstract

The study was undertaken to evaluate the therapeutic efficacy of Escherichia coli LPS and Neem oil in 40 infectious repeat breeder cows. The animals were divided in 2 different groups viz. Group-I 20 and Group-II 20 treated with E. coli LPS @ 100 mcg/ml activity in 30 ml sterile PBS and neem oil @10 mcg/ml in 30 ml distilled water in intrauterine route, respectively. The diagnosis was confirmed by estimation of pH, PMN cell count, White Side Test and Spinnbarkeit test, which indicated 93.00% cows having alkaline pH, PMN cell count >5% and all cases positive for White Side Test. Recovery rate after treatment were found as 16 (80.00%) and 15 (75.00%), respectively. The conception rate was found as 14 (73.68%) and 12 (63.15%) in cows in Group-I and II, respectively. On conclusion, cows treated with E. coli LPS showed higher recovery rate.