In-vitro assessment of anti-gingivitis effect of homeopathic ingredients based toothpaste

Abstract

Aim: The aim of this study was to perform In vitro assessment of Homeopathic ingredient based toothpaste against oral disease causing pathogens Porphyromonas gingivalis (P. gingivalis). Objective: The present study was designed to evaluate in efficacy of the herbo-mineral toothpaste composition prepared using certain homeopathic medicinal plant extracts & Zinc Salt for the protection of oral hygiene and prevention of gum diseases by inhibition of disease causing microorganism P. gingivalis. Methods: The homeopathic ingredient based toothpaste was made of Zinc Salt & Hekla Lawa Extract fine ash from mount Hekla Lawa, Hamamelis virginica Extract, Plantago Major Extract, Phytolacca decandra Extract and Calendula officinalis Extract combined in Toothpaste formulation. The product was tested against microorganism’s P. gingivalis using agar well diffusion method. The agar-well diffusion method was used to test the antimicrobial effect. Inhibition zones formed around toothpastes after 24 hours of incubation were measured and the data collected were statistically analysed. The time-dependent killing assay was carried out on P. gingivalis. Conclusions: In vitro assessment of homeopathic ingredient based toothpaste against disease causing oral pathogens revealed the efficacy of toothpaste against major gingivitis causing oral pathogen P. gingivalis.