in vitro callus induction of safflower (Carthimus tinctorius L.)

Abstract

In present study efforts were made to improve to study invitro callus induction of safflower. Safflower local genotype was used to study in vitro callus induction from leaf explant. Safflower seed were in vitro germinated on plane MS media. The leaf explants were excised from it aseptically cultured on MS media with different concentrations of 2, 4-D and incubated at 25 ± 2°C in light condition. Good callusing was observed on MS media containing 3.0, 3.5, 4.0, mg / l 2, 4-D within 3-4 week, yielding white embryonic and globular shaped calli. Profuse callusing was obtained on MS media containing 3.5 mg / l 2, 4-D.