In vitro analysis of antimicrobial activity in exposed collagen membranes applying chitosan-metronidazole in topical gel

Abstract

Guided Bone Regeneration through the use of a membrane has been a Gold standard to obtain the best results, in turn, being a sensitive technique whose main complication is its exposure and infection by pathogenic periodontium bacteria. Objective: To observe the antimicrobial effect of topical chitosan-metronidazole on Porphyromona gingivalis. Materials and Methods: Topical Chitosan-Metronidazole was prepared with which it is antimicrobial sensitivity was observed in the crop medium of Müller-Hinton agar with the well diffusion method after having applied 50 microliters of P. gingivalis, 8mm was extracted with a punch and filled with chitosan-metronidazole, the inhibitory halo was analyzed to obtain results. Results: The inhibitory halo was observed and measured in the two samples which is 20 mm for both, obtaining a sensitive result. Conclusion: Topical chitosan-metronidazole was shown to have a good effect on P. gingivalis.