Genetic Polymorphism in Honey bee Apis cerana indica (F.) (Hymenoptera: Apidae) by Random amplified polymorphic DNA (RAPD) Markers

Abstract

For pollination purpose and apiary by-products production Apis cerana indica is highly demanded honey bee in India. Honey bee is a polymorphic bee having three casts queen, workers and drones. In Apis cerana indica queen honey bee undergoes multiple mating with drones to gather genetic diversity in colony. Genomic DNA sample of adult drone and worker honey bees was used to study genetic polymorphism by Random amplified polymorphic DNA (RAPD) markers. Ten Primers of each containing different 10mer oligonucleotide sequence were used to generate RAPD profile. Present study observed the banding pattern for presence and absence of characters and find out the polymorphism information content (PIC) value generated by each primer. Low level of genetic polymorphism was found between workers and drones with 0.34984 PIC value.