HR-LCMS analysis of tuber and callus of Ceropegia bulbosa roxb. var. bulbosa

Abstract

Ceropegia bulbosa var. bulbosa is threatened medicinal plant belongs to family Asclepiadaceae. Present analysis is based on most reliable analytical procedure, High Resolution- Liquid Chromatography Mass Spectrometry (HR-LCMS) to detect bioactive phytoconstituents from in vivo tuber and in vitro callus of Ceropegia bulbosa. The present study provides evidence that ethanol extract of Ceropegia bulbosa Roxb. var. bulbosa tuber showed presence of 1, 4-Dimethylpyrrolo [1, 2-a] pyrazine, Gabapentin, Americine, Oseltamivir, 4-Methylphenyl dodecanoate, 17 beta-Methylestra-1,3,5(10)-trien-3-ol, Citronellyl hexanoate, Limonoate, 1-Monopalmitin, Mitoxantrone, Butyl dodecanoate compound. In vitro callus showed presence of Elaeokanine C, Validamycin A, Flurazepam, Isopentenyl adenosine, Mitoxantrone, Phytosphingosine, Oxidized dinoflagellate Luciferin, Harderoporphyrinogen, 6-Deoxocathasterone, Irinotecan, Mycinamicin VII compound. It is observed that, the compounds present in in vivo tuber were also present in in vitro callus. Some variation was observed in some compounds like Validamycin A, Flurazepam found in callus which were absent in tuber.