SDS-PAGE analysis of hormone treated larvae of honeybee Apis cerana indica (F.) (Hymenoptera: Apidae)

Abstract

SDS-PAGE analysis of protein was carried out in fifth instar larvae of worker and drone caste at 24 hrs and 36 hrs treatment with acetone (control), JH-III and 20-HE. 52kDa to 106 kDa protein bands were observed in both caste of larvae after JH-III, 20-HE and acetone treatment after 24 hrs and 36 hrs. Female specific 170kDa vitelogenin like protein band was observed in larvae of both caste. Caste specific protein band 191kDa and 32kDa was observed in worker larvae and 38kDa protein band was observed in drone larvae. This difference is due to different developmental timing and difference in physiology of both caste.