Effect of plant growth regulators on growth of callus of Decalepis hamiltonii

Abstract

Decalepis hamiltonii is an medicinal plant with different biological activities. Present study involves callus growth in this plant using different plant growth regulators. Producing callus cultures is a very important for production of secondary metabolites. Callus cultures were developed by using the media Murashige and Skoog (MS), supplemented with 2, 4-D, BAP and combination of 2, 4-D+Kn, BAP+GA3, NAA + Kn, 2, 4-D+IBA. The callus development was estimated by its fresh, weight, dry weight and the morphology.