The Bio-efficacy of Inesfly vesta 50 (Transfluthrin 0.5% w/w) insecticidal paint: An alternative vector control intervention against Anoheples gambiae mosquitoes under laboratory condition, Keffi, Nigeria

Abstract

Malaria remains a major public health concern. Particularly in Nigeria where more than 80% of the cases are reported and locally among natives in Nigeria. There are serious indication of insecticide resistance in Nigeria. Under PMI/Vectorlink laboratory condition, cement plaster surface and wooden board were brushed with a primer within 24-48hours before the paint application. The inesfly vesta 50 (transfluthrin 0.5% w/w) insecticide paint was applied at the dosage of 8m2/L for cement and wooden board drying at room temperature for 5 days. The plastic cones were fixed on the treated walls and wooden board at 0.5m, 1.0m, and 1.5m parameters height, after which 10 non-blood fed females mosquito were gently introduced into the cone chamber for 30- 60 minutes observation. The holding paper cups were kept under favourable temperature of 27oc and humidity at 70%. In a dose trial finding, the data obtained were analyzed in IBM SPSS version 23.0 using ANOVA and the results presented as mean ± standard deviations of triplicate observations showed 100% yield (100.0±0.00*) mortality of malaria vector of novel trial with 0.5% w/w transfluthrin painted substrates in 24hours post exposure to 6months post application, except that, at l.5m in the month of July, 98% knockdown/mortality was recorded which follow same sequence of method and procedure in the application on the substrate absorbency, though, results showed high level of susceptibility from February to July (100% mortality rate). Results from wall cone and wooden board bio-assay residuality was alarming, with very high kill rates observed after 24hours post exposure. From the treated bio-assay of inesfly vesta 50 (Transfluthrin 0.5% w/w) by possible means showed that, this formulation is effective on malaria vector and possibly other insects. This suggests that, the transfluthrin 0.5% w/w can be tried alongside other known application with organophosphate, pyrethroids and the carbamate group, and can reduce human mosquito contact and impact on the perennial malaria transmission.