Antiprotozoal activity and phenolic constituents of ethyl acetate extract from Parinari excelsa sabine (Chrysobalanaceae)

Abstract

Parinari excelsa Sabine (Chrysobalanaceae) is a plant used in Ivorian traditional medicine to treat various diseases including malaria. Present study aims to determine the antiprotozoal activity of the ethyl acetate extract from the trunk back and its many phenolic constituents. The ethyl acetate extract was fractioned by column chromatography method using silica gel and the obtained sub fractions were evaluated in vitro against Trypanosoma cruzi, Leishmania donovani Axen and Plasmodium falciparum K1. The phytochemical screening of the ethyl acetate extract (EA) led to the identification by semi-preparative HPLC method of twelve known phenolic compounds: Kaempferol (1), Apigenin (2), Hydroxybenzoic acid (3), Procyanidin B1 (4), Protocatechuic acid (5), Procyanidin B2 (6), Ferulic acid (7), Xanthotoxin (8), Chlorogenic acid (9); p-Coumaric acid (10), Scopoletin (11) and Quercetin (12). Biological study revealed height antiplasmodium, antitrypanosome and antileishmaniasis activities of ethyl acetate extract and its sub-fractions.