Antioxidant activity and Phytocompounds from Rogeria adenophylla J. Gay crude extract

Abstract

Rogeria adenophylla is a medicinal plant widely used in ethnomedicine for the treatment of inflammations, infectious diseases, wounds and as an antinociceptive. Therefore, there is need to investigate its antioxidant property and identify some of its phytocompounds. The crude extract of R. adenophylla was obtained using maceration technique. The extraction solvent was removed using rotary evaporator at 45 oC to yield a brown residue. The antioxidant activities of fractions were assessed using DPPH while isolation of compounds was achieved on open column chromatography and coded A1 and A3. The characterization of all isolated and purified compounds were carried out using IR, NMR (1D and 2D) and GC-MS data. The study revealed that the ethanol leaf extract possessed the highest antioxidant property of about 87.17% while that of ascorbic acid standard was 75.40%. Compounds A1 and A3 were identified as stigmasterol and palmitic acid methyl ester based on the analysis of all spectroscopic data and in comparison with literature. The presence of these phytocompounds and the observed antioxidant activity justify the use of R.adenophylla in ethnomedicine.