Comparative study of five culture media for in vitro culture of Trichomonas gallinae from birds in Assam

Abstract

A study was carried out in order to compare the in vitro efficacy of five different culture media against the flagellate protozoa, Trichomonas gallinae. For in vitro culture, oral swabs of T. gallinae were collected randomly from oropharynx and crop after visual inspection of birds as pigeon, duck, chicken and quail with a sterilized cotton swab and cultivated in five media namely modified Diamond media, Medium199, Minimum Essential Medium (MEM), RPMI 1640 and Nutrient broth. The initial inoculation of trophozoites into each media was 1X103cell/ml. Among all the five media, Medium 199 showed the highest growth rate and motility of the organisms till 7 days after initial inoculation. All media were found suitable for harvesting and sub culture of T. gallinae under in vitro condition.