Polymorphism of DNA microsatellite BMS 1248 and ILSTS 006 on taro white cattle in Bali, Indonesia

Abstract

The population of Taro white cattle is only about 59 cattle. This small population which will result in the occurrence of inbreeding. This can lead to loss of genetic diversity. The purpose of this research was to characterize Taro white cattle breed using microsatellite BMS1248 and ILSTS006. A total of 20 Taro white cattle were blood drawn and extracted, and genome DNA were amplified by PCR. The result showed that the markers successfully amplified in 18 Taro white cattle. Microsatellites locus BMS1248 and ILSTS006 were shown to be highly polymorphic. In locus BMS1248 were found 5 alleles with allele’s size ranged from 139 to 165 bp. The number of alleles in locus ILSTS006 were found 5 alleles with alleles size ranged from 162 to 168 bp. The average observed and expected heterozygosity were 0.25 and 0.75 (BMS1248) and 0.5 and 0.671(ILSTS006). The PIC value was 0.679 (locus BMS1248), and 0.58 (locus ILSTS006).