Effect of garlic extract loaded on zinc oxide nanoparticles against Sarcocystis in vitro

Abstract

The present study aimed to investigate the effect of garlic extract loaded on zinc oxide nanoparticles on the viability of Sarcocystis in vitro Samples of the parasite in the study was divided into three group inside to control group each one consists of 20 parasite in petri dish (5 petri dish) for each concentration contain the Phosphate Buffer Saline(PBS). The group treated with one concentration of the hybrid nanoparticles (Garlic extract loaded on zinc oxide nanoparticles) (0.1, 0.3, 0.5) mg/ml in 4 ᵒC and noticed the viability for five days. The nanoparticle measuring by SEM (Scaning Electronic Microscop) in Karbala University, College of Veterinary Medicine, the results showed that process of loading garlic Extract on the surface of zinc oxide nanoparticles was succeed and the hybrid nanoparticles had great effect (100%) in mortality of the parasite in high concentration 0.5 mg/ml followed by 0.3 mg/ml (8.98%) and (17.66%) in 0.1mg/ml. The statistical analytic results showed that there was significant increasing in mortality in all concentrations.