Comparison of a commercial and a manual antigen coated ELISA tests used in detecting antibodies against Japanese encephalitis virus in pig serums collected from the Province of Bali

Abstract

The most common serological test perform for screening the antibodies against JEV is enzyme-linked immunosorbent assay (ELISA) and it has been commercially available recently. However, the commercial test tends to be expensive to be used for screening masses samples in developing countries. Then, a manual antigen coated ELISA test was prepared to meet the situation. This study aim was to compared a commercial and a manual coated ELISAs in detecting the antibodies against JEV in pig serum samples collected from Bali, Indonesia. A commercial ELISA was used as a reference to assess its relationship with the manual coated ELISA. A two-by-two table of the two tests’ results was set followed by the calculation of a Kappa agreement to determine the relationship of the tests. The results showed a substantial agreement with value of 0.65. However, this substantial value indicated both the tests was satisfactory indicating the manual coated ELISA had similar results compared with the commercial test. Therefore, the precision and validity of tests, especially for the manual coated ELISA need to be evaluated further to ensure it's a good diagnostic essay. In more specific, it can be used to regularly detect antibodies in large amount of the samples in endemic areas of developing countries.