Biometric analysis on three offspring of transgenic mutiara catfish (Clarias gariepinus)

Abstract

This research was conducted at the hatchery and the Laboratory of Biotechnology of the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran from October to December 2021, with the aim of detecting deformities and phenotypic character abnormalities in G1, G2, and G3 transgenic mutiara catfish (C. gariepinus). The study used a completely randomized design with four treatments (G1, G2, G3 as transgenic mutiara catfish, and non-transgenic mutiara catfish as controls) with three replications. Each treatment used 10 fish (test sample). The results showed the phenotypic characters of body weight (BW), total length (TL), standard length (SL), head length (HL), head width (HW), dorsal length (DL), pectoral length (PCL), and caudal length. (CL) inheritance in G1, G2 and G3 was asymmetric (SD ˃ 1.0). All biometric characters in growth control fish were symmetrical, indicated by SD values ​​< 1.0. Qualitative biometric analysis showed that abnormal fish (body defects) were only found in two fish in G2 and one fish in G3 from a total sample of 10 fish. However, 1 non-transgenic (control) fish was also found. Overall, these defects were only found on the pectoral fins, both in transgenic and non-transgenic catfish, and had little effect on fish growth (still normal).