Molecular detection of 2c variant of Carnivore protoparvovirus1 from Mizoram, India

Abstract

Canine parvovirus2 (CPV2) is associated with acute fatal hemorrhagic diarrhea in pups. The present study was conducted to detect the variant of Carnivore protoparvovirus 1from a case of severe hemorrhagic gastroenteritis in a pup by polymerase chain reaction (PCR) combining with partial VP2 gene sequencing. PCR was performed, targeting a partial segment of the VP2 protein gene, which gives an amplicon of 536 bp. PCR amplicon was cloned and sequenced. Phylogenetic analysis was performed by comparing sequences with all other variants of CPV2 viruses, revealed that the virus belonged to the CPV2c variant (MN218607). The partial VP2 gene sequence revealed a 99.4 to 99.6% sequence similarity with other reported CPV2c variant. Typical substitution of Glutamine (E) instead of Asparagine (N) in the amino acid position 426 could be observed, further confirming the CPV2c variant. Till now, there is no published report available that show the presence of CPV 2c variant in the dog population of Mizoram.