Effect of repeated oral administration of butachlor and lead alone and in combination on oxidative stress and histopathology in Wistar rats

Abstract

The experiment was carried out to evaluate the rising concern over the additive effect of one chemical with another chemical, which has raised a lot of health concerns in the general population. In the experiment, adult wistar rats of either sex were divided into 4 groups and the study was conducted for a period of 28 days. Group I served as control and was provided with water only for drinking purposes. Animals of group II were administered butachlor@262mg/kg B.W/P.O. Group III received administered lead acetate@265ppm orally, while animals of group IV received a combination of butachlor@262mg/kg and lead acetate@265ppm. Oxidative stress was revealed by significantly increasing lipid peroxidation and decreased blood glutathione, glutathione peroxidase and transferase, catalase and superoxide dismutase in blood and tissues. Histopathologically, the liver and and kidney exhibited structural alterations as compared to control. The study concluded butachlor and lead produced a more deleterious systemic effect in combination than alone (additive toxic effect).