Preliminary studies into transmogrification of Aspergillus genome for over expression of cellulase

Abstract

The aim of this investigation was achieved by exposing native Aspergillus niger (WldAN) to an ultraviolet (UV) germicidal lamp (256 nm) kept at a distance of 40 cm for a time interval ranging from 2 –14 minutes, in a total of 56 minutes and finally using the improved strain to synthesize a more stable cellulase. The results showed growth improvement of the modified isolate with a density of 3.54 g/ml at the 9th hour as compared to 3.36 g/ml density of the native strain at the 7th hour on the 3rd day. Cellulase produced by submerged fermentation at 25 oC for 4 days using the native Aspergillus niger (WldAn) and modulated Aspergillus niger (UVAn) respectively recorded activities of 3.50 U/ml at 60 oC and 2.85 U/ml at pH 4.0 as compared to 2.40 U/ml at 50 oC and 2.24 U/ml at pH 5.0 recorded for cellulase synthase from the native strain