Deciphering in vitro mutated sugarcane clones for growth parameters

Abstract

Callus derived from sugarcane variety CoN – 13073 subjected for mutagenic treatment varying in different concentration as well as time period. Callus culture of 25 - 30 days old white globular form is considered a suitable material to undergo mutagenic treatments. Among the mutagenic treated calli, Maximum shoot length (26.6 cm) was observed in the treatment, EMS 0.2% + 30 min. Maximum root length (12.8 cm) was observed in treatment MMS 0.3% + 30 min. A higher concentration of all mutagenic agents registered higher root length with the maximum of 1.14 g was observed in treatment EMS 0.3% + 60 min. Maximum leaf area (44.7 cm2/plant) was observed in treatment MMS 0.2% + 30 min. Irrespective to concentration SA and EMS found significant for this character. Maximum chlorophyll content index (4.87) was observed in treatment EMS 0.2% + 30 min. In case of this character higher to lower concentration as well as time period influenced in both higher and lower directions. Among the treatments, the maximum multiplication rate (12.45%) was observed in treatment EMS 0.2% + 30 min. Both factors showed different effects on this character. The effect of the mutagenic agent was found to be succeeding to create variability at a lower concentration for lower time intervals for most of the characters under study. In the present study EMS, 0.2% in addition to 0.3%, treatments were most effective. And the effectiveness decreases with the increase in the time interval.