Role of biochip indirect immunofluorescence assay in diagnosis of autoimmune vesicobullous disorders in a tertiary care setting

Abstract

Aim: To evaluate the role of BIOCHIP mosaic based immunofluorescence test in the screening and diagnosis of autoimmune bullous disorsders. Results: Results of DIF and BIOCHIP were compared with clinical diagnosis. The sensitivity of DIF was 99% in all epidermal and subepidermal bullous diseases. The sensitivity of BIOCHIP in the diagnosis of pemphigus vulgaris, bullous pemphigoid, pemphigus foliaceus was almost 100% and comparable with that of DIF. Conclusion: Thus the sensitivity and diagnostic accuracy of BIOCHIP is comparable with DIF making it a more effective practical screening tool for patients with suspected AIBD. Thus, BIOCHIP can be used as first line tool in the diagnosis of autoimmune bullous disorders.