Assessment of molecular diversity among mungbean [Vigna radiata (L.) Wilczek] genotypes using EST-SSR marker

Abstract

Genetic diversity can be assessed through molecular markers at the DNA level because of their abundance as compared to morphological characters and independence of environmental influences. By using expressed sequence tag-simple sequence repeat (EST-SSR) markers molecular diversity of thirty mungbean genotypes was analysed at S.D.A.U., Sardarkrushinagar (Gujarat) during 2019. DNA was extracted using CTAB method. Total 21 EST-SSR primers were screened across all the genotypes. Amplification of eleven alleles were done and high polymorphism (100.00%) was found from 5 EST-SSRs primers (MB21347, MB11596, MB34120, MB24478 and MB15212). Each locus has 2 to 3 alleles, with 2.2 average alleles per locus. The range pf PIC (Polymorphic Information Content) was 0.204 to 0.580, with 0.348 average. Similarity indices derived from Jaccard’s similarity co-efficient were estimated on the basis of five EST-SSR primers ranged from 0.091 to 1.00. Genotype, LM-584 and PIMS 1 showed minimum similarity. Two main clusters and six sub-clusters were formed in a dendrogram prepared using UPGMA analysis. PCA and PCoA plot based on EST-SSR primer study was constructed and genotypes have been distributed into four major groups in each method. Overall results suggested that EST-SSR can be a great tool to explore genetic diversity in mungbean.