Safety and immunogenicity evaluation of Brucella abortus S 19 reduced dose vaccine in comparison with Brucella abortus S 19 standard dose vaccine in cattle

Abstract

Bovine brucellosis, caused by Brucella abortus, is a serious zoonotic disease manifested by reproductive disorders in animals and results in huge economic losses to dairy farmers. This study was undertaken to evaluate the safety and immunogenicity of Brucella abortus S-19 reduced dose vaccine in comparison with Brucella abortus S-19 standard dose vaccine in 4-10 months old female cattle calves by measuring humoral immune responses and cell mediated immune responses. The humoral antibody responses in reduced dose group indicated absence of persistent antibody titres, whereas, those vaccinated withBrucella abortus S-19 standard dose vaccine showed persistence in antibody titres till day 240 with i-ELISA and day 150 with c-ELISA post vaccination. The IFN-γ responses in vaccinated groups were found to be diminishing till 3 years post vaccination. It is concluded that Brucella abortus S-19 reduced dose vaccine performed similar to that of Brucella abortus S-19 standard dose vaccine.