Surface spreading sporulation (SSS) technique for microfungal purification

Abstract

A technique was used for the purpose of separating microscopic filamentous molds. The primary aim was to establish a method that will conveniently distribute vegetative spores across the surface of a culture medium allowing easy detection of desired strain. A contaminated Sabouraud Dextrose Agar (SDA) cultured plate previously used for isolation at 25 oC after 5 days was used for the purpose of this investigation. The culture plate showed a mixture of probable isolates after incubation period signifying a case of cross contamination. Following standard laboratory procedure, the following isolates: Aspergillus Niger, Aspergillus terreus, Penicillium funiculosum and Penicillium chrysogenum were recovered using the surface spreading technique. The investigation confirmed the level of microbial co-existence which is often difficult to manage in cases of pollution caused by molds due to their growth pattern. On the other hand, the practise provided a simple and effective application in handling purification of multicellular sporulation.