Determination of effective dose of gamma irradiation for lentil seed mutagenesis

Abstract

Mutation breeding is key method for generating huge number of variation. In less diverse crops lentil has potential for generating variation. Effective dose need to standardize for inducing variation. Gamma irradiation using standard LD50 dose will generate sufficient variation undergone several mutation. This study identifies the actual effective LD50 dose by evaluating several dosage of irradiation and considering morphological effects of physical irradiation. Result of this study revealed that 300Gy is the effective LD50 for giving irradiation to lentil seeds. Effective dose for irradiation will help to develop desirable mutation in succeeding generation after irradiation treatment.