Isolation of Mycoplasma gallisepticum from the natural cases of chronic respiratory disease

Abstract

Present work is a postmortem study. Total 428 dead birds, suspected to have died of chronic respiratory disease were considered for sampling. Swabs collected from the respiratory organs of the chickens and subjected for culture of the organism. Collected swabs were inoculated into PPLO broth medium allowed to incubate for 2-3 days. After removal of the contamination, culture from broth medium were inoculated over enriched PPLO agar plate. Typical “fried egg” like colonies appeared after 7 days of incubation. Colonies were confirmed as Mycoplasma gallisepticum (MG) by PCR. MG has been proven to cause serious respiratory illness in the chickens under present investigation.