Identification of limonoids and steroids from the leaves of Turraea heterophylla (Smith) by HPLC‐ESI‐QTOF‐MS/MS methods

Abstract

Turraea heterophylla (Smith) is a species used in Ivoirian medicine as antimalarial, aphrodisiac and various human diseases. Using the dereplication method, the analysis of the crude methanol extract of the leaves led to the identification of six compounds including three limonoids (28-nor-4α-carbomethoxy-11β-acetoxy-12α-(2-methylbutanoyloxy) 14,15- deoxyhavanensin-1,7-diacetate; mzikonol; 1,2- dihydro-azadirone) and three steroids (3ß-acetoxy-5-pregn-7,20-dien-6-one; 3β-hydroxy-5α-pregn-6-one; villosterol). These secondary metabolites, which are reported for the first time in T. heterophylla, were identified according to their HPLC‐ESI‐QTOF‐MS/MS data.