Study of quantitative traits locis for grain characters in double haploid population of Rice

Abstract

A double haploid mapping population consisting of 75 lines of a cross between the irrigated indica variety IR64 and the upland japonica variety Azucena was used in the present experiment. The complete set of double haploid lines along with parents was evaluated under two sets of conditions, 1. Transplanted with water stress and 2. Transplanted with no stress over two years. Water stress was imposed at flowering and observations were recorded for two traits. A total of 10 putative QTLs for 100 grain weight, grain length, grain breadth and length and breadth ratio were detected, which were found to be explaining a minimum of 9.8 per cent to maximum of 19.3 per cent of phenotypic variation, individually. These QTLs were mapped on the rice genome and linked DNA markers have been validated for use in the marker assisted breeding program.