Effect of hyper active amylase produced by Bacillus species consortium

Abstract

An investigation was carried out to determine the effect of amylase activities from Bacillus licheniformis (Bl), Paenibacillus polymyxa (Pp) and a consortium of both (BlPp). The results showed growth density of 3.58 g/ml and 3.38 g/ml for (Bl) at 9th hour and (Pp) at 7th hour respectively. Amylase activities of 0.45 U/ml and 0.43 U/ml were recorded at optimal pH 7.0 for Bi and Pp, while an increased activity of 0.55 U/ml was recorded for the consortium of (BlPp) at optimal pH 5.0. Similarly, secretome activities were recorded at optimal 50oC with values of 0.48 U/ml and 0.40 U/ml for (Bl) and (Pp), while a higher activity of 0.56 U/ml was observed for the consortium of (BlPp). Although there have been various developments of synthetic co-cultures, the results from this experiment have shown a fundamental and unique approach of natural microbial consortium application for synthesis of refined industrial byproduct.