Polymorphism in x-region of spa gene in S. aureus isolates from goats with clinical mastitis

Abstract

Staphylococcus aureus is the most frequently mastitis causing bacterial pathogen in dairy milch animals including goats. In the present investigation 34 S. aureus from goat mastitis were characterize for x-region of spa gene where in amplification of x-region of spa gene produced amplicons in 32 isolates of with specific primers whereas two did not produce any amplicon which were considered spa negative isolates. Of the 32 isolates 23 produced single amplicon, 8 produced double amplicons and one isolate produced three amplicons. All the 32 isolates were divisible into 19 spa types and the size of amplicons obtained were 80, 200, 220, 240, 250,260, 280, 300, 320 and 340bp with a calculated number of repeats of 3, 3, 3, 5, 1, 3, 2, 4, 2 and 2, respectively.