Study of Passiflora incarnata Alone and with Asparagus Racemosus for Anxiolytic Activity in Mice

Abstract

In this study, we have evaluated anxiolytic activity of treatment of Passiflora incarnata alone and with Asparagus racemosus in mice, to find whether the treatment produce synergistic anxiolytic activity or not. Anxiolytic activity was evaluated using marble bury test, elevated plus maze and effect of drugs on GABA levels in mice brain using UV-Vis spectrophotometer. Drugs were administered orally as per mg/kg body weight, one time a day for 10 days. Methanolic extract of Passiflora incarnata leaf-stem (MEPILS) and Methanolic extract of Asparagus racemosus roots (MEARR) was used as test drugs. Lorazepam 0.05 mg/kg was used as standard drug. In marble bury test and elevated plus maze, treatment of MEPILS with MEARR showed significant synergistic anxiolytic activity in mice. Increase in GABA levels in mice brain after the treatment of MEPILS with MEARR was observed. This study suggests that treatment of MEPILS with MEARR produces significant synergistic anxiolytic activity in mice.