Evaluation the in vitro antibacterial activity of acetone leaf extracts from Pistacia lentiscus against multi-drug resistant Pseudomonas aeruginosa and Staphylococcus aureus

Abstract

Since ancient decades, Pistacia lentiscus is known by its immense medicinal qualities. The leaves of the Pistacia lentiscus plant possess a multitude of biological activities, such as antioxidant, antimicrobial, antitumor, anti-inflammatory, anticancer, antiradical, etc. The present study was conducted to evaluate the phytochemical proprieties, mainly presented by the presence of pyrogallol and qurecetin, which considered as the most important bioactive compounds with antioxidative and antimicrobial properties and then assess the antibacterial activity of acetone leaf extract of Pistacia lentiscus on two pathogenic bacteria namely Staphylococcus aureus and Pseudomonas aeruginosa using agar well diffusion method. Our results indicated that the total contents of pyrogallol and qurecetin from the acetone extract of Pistacia lentiscus leaves were gradually increased with increasing concentrations, which obviously appeared as a positive and highly significant linear correlation between absorbance and concentration (pyrogallol / qurecetin: Coefficients of determination (R2) = 0.9908 / 0.9978, respectively). The results of the present work revealed that at all concentrations, the acetone leaf extract of P. lentiscus recorded different degrees of antibacterial activity against both bacteria. Therefore, we can conclude that the antibacterial activity of the plant under investigation may be as a result of the bioactive compounds present.