Isolation and identification of rumen microbes and rumen fluid enzymes to use as the bio-degradator feed in aquaculture

Abstract

The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen cattle and proteolytic enzymes from the rumen sheep fluid. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria and biodegradation by proteolytic enzymes that act to degrade feed fibrous material to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzymes. The cattle rumen fluid were used to identify the rumen microbes. Based on the cellulolytic analysis through Iodin test, two bacteria isolates were selected and showed the highest score of cellulolytic index scored of 3.0 and 3. Morphologically, the microbes identified as Bacillus megaterium and Bacillus mycoides and using KitStandard Analytical Pofile Index (API). In another side, the sheep rumen fluid were extracted to analyzed the enzymes activity and showed the rumen enzyme activity obtained in this study resulted much higher cellulase activity of 1.66 IU / ml / min than other enzymes such as amylase, phytase, and protease. The present study showed that rumen fluid has its possibility as bio-degradator of feed materials in aquaculture, which contain high level of cellulose.