Cytological aspects of Culex (Culex) pipiens Linn., from Manipur India

Abstract

The cytological studies on mosquito is a much to ascertain the systematic conflicts and as well as to adopt the materials in the regular practical classes in educational institutes. The chromosomal compliments, salivary gland and polytene chromosomes are yet to study for Culex (Culex) pipiens Linn., the larvae were collected on 27th June, 2021 from the locality and identity was compared with available literatures and published papers for adult, pupa and larvae. The larvae were dissected to harvest the salivary gland and imaginal discs (IDS) in hypotonic solution. The salivary gland consists of 8 (eight) small lobules. The salivary glands were fixed in Carnoy’s Fluid for 10 minute and stained in 2% Acetocarmine solution and observed under microscope. The three elongated polytene chromosomes were observed. The IDS chromosomes comprises of one pair of submetcentric and two acrocentric pairs. The number of 2n=6 and three elongated polytene chromosomes are quite in accordance with published works but the types of the chromosomes are quite different that might be due to chromosomal variation or might be other reasons. In future, more inclusions of habitats and more materials will be required to ascertain the validity of the species C. (C).pipiens from Manipur.