Evaluation of immunomodulatory cells CD4+and CD8+and their ratio using alcoholic seed extract of Trigonella foenum graecum and alcoholic leaves extract of Coccinia indica by flow cytometry in streptozotocin-induced diabetic rats

Abstract

The present study was taken up to evaluate the immunomodulatory efficacy of Trigonella foenum graecum and Coccinia indicaindividualy and in combination along with glibenclamide in streptozotocin induced diabetic rats for a period of 90 days. The various groups in this study included normal control (Group-I), diabetic control (Group-II), diabetic rats treated with glibenclamide (Group-III), diabetic rats treated with Trigonella foenum graecum (Group-IV), diabetic rats treated with Coccinia indica (Group-V), diabetic rats treated with Trigonella foenum graecum and Coccinia indica (Group-VI), diabetic rats treated with Trigonella foenum graecum and glibenclamide (Group-VII), diabetic rats treated with Coccinia indica and glibenclamide (Group-VIII). Diabetic rats treated with Trigonella foenum graecum, Coccinia indica and glibenclamide (Group-IX) respectively. There was significant variation in CD4+ and CD8+ values and in their ratio in diabetic rats when compared to normal control rats. The higher alleviation of the CD4+ and CD8+ values and in their ratio was observed in group which received combine Trigonella foenum graecum and Coccinia indica extracts. The groups which received individual extracts were showed moderate alleviation in CD4+ and CD8+ values and in their ratio whereas Group treated with glibenclamide did not show any significant improvement in the CD4+ and CD8+ values and in their ratio.