Spectrophotometric method of Ascorbic acid in Carica papaya L. extracts: An in vitro study

Abstract

Carica papaya L. is one of the precious plants used for different use in medicinal herbalism for contagious diseases. Carica papaya leaves, fruit and seeds are used as complementary alternative ethano medicine. Fresh leaves samples of the plant were collected during the month of June 2019 from different areas of Perambalur district, Tamil Nadu, India. The purpose of the study was to evaluate the biochemical composition in leaves of Carica papaya growing in the Semi-arid region of Perambalur, Tamil Nadu and based on the result to validate its consequence in relation to chemoprevention of infectious diseases. The dried leaves were further analyzed for biochemical constituents like ascorbic acid and Cystatin using different solvent extraction. The result shows the leaf extract of C. papaya has high permeability of ascorbic acid and Plant Cystatin, phenols, flavanoids and saponins possess significance at α=0.05 in methanol, chloroform, ethyl acetate and water extraction. The high amount of total phenolics, saponins and total flavonoids in the ethyl acetate and methanol fractions show their permeability to their antioxidant activities. Papaya leaves contain vitamin C has a therapeutic effect in curing the skin rejuvenation which also supports the immune system and digestive ailment.