Protocol for DNA extraction and molecular characterization in medicinal plant Carica papaya L. varieties

Abstract

Carica papaya L. shows considerable phenotypic variation in many horticultural traits like, fruit size, fruit shape, flesh color and sweetness. In present study, six varieties were differentiated with the help of molecular marker technique. Genomic DNA was isolated from these six varieties using standardized DNA isolation protocol. For molecular characterization, total 30 different primers of ISSR were tested for all six varieties. The PCR amplification results obtained using ISSR primers for all six varieties which showed unique band patterns on agarose gel electrophoresis. A statistical analysis of the monomorphic and polymorphic bands of the all varieties was performed. Among 30 primers, 24 primers given high polymorphism and proved the best to distinguish six varieties from each other. It is observed that ISSR technique is a useful molecular marker to distinguish Carica papaya varieties for best medicinal formulations and for clonal selection.