The rate of embryonic development and hatching in transgenic G3 mutiara catfish (Clarias gariepinus) eggs at room temperature

Abstract

Mutiara catfish is one of the most important catfish strains developed in Indonesia. However, the growth stability of cultured non-transgenic Mutiara catfish fluctuates. This problem can be solved by developing transgenic Mutiara catfish (containing the African catfish growth hormone gene, CgGH) to improve the stability of fish growth. Controlled handling of eggs is carried out to reproduce this superior fish in each generation. The egg handling in a controlled system was performed to reproduce this superior fish in each generation. The G3 transgenic Mutiara catfish eggs production was carried out with three different broodstock pairs (pairs A, B and C). The results of observations of the rate of embryogenesis and hatching of the G3 eggs showed that eggs from pair A were faster than B and C. Transgenic catfish eggs hatch 1-1.5 hours faster than non-transgenic fish eggs at room temperature.