Evaluation of anti-lipid peroxidation and cytotoxic activity in the methanolic extracts of Hibiscus and Amla

Abstract

Hibiscus and Amla are the most locally available medicinal plants in India. Hibiscus flowers and leaves are used as skin and hair products, and Amla fruit has been operating as a traditional medicine to treat many diseases. Both plant materials have an excellent value in the field of ayurvedic medicines in India. In this present study, Hibiscus and Amla were tested for anti-lipid peroxidation and cytotoxic activity. The extracts were prepared by the solvent extraction method, and Methanol was used as the solvent. The egg yolk was used to evaluate the anti-lipid peroxidation, TBARS assay, and the in-vitro cytotoxic study performed against UMR 106 cell lines, and MTT was used to estimate the cell growth. The result of the TBARS assay for the Amla and Hibiscus methanolic extracts had shown IC50 values of 3.97µg/ml and 7.03µg/ml, respectively, in which Amla has shown significant results when compared with Hibiscus. In the development of MTT assay, sample Hibiscus showed an EC50 value of 64.74% proliferation in UMR106 cells, whereas Sample Amla did not show significant proliferation; hence EC50 was not calculated. The result concludes that the Amla has good anti-lipid peroxidation activity, whereas the Hibiscus flower has less cytotoxic property.