Modulatory effect of combination of green tea with various herbs on angiogenesis and on urolithasis

Abstract

Green tea is one of the highly consumed drinks throughout the world after water and has gained popularity due to its various health benefits. The objective of this study was to investigate the regulatory effects of green tea on angiogenesis and on urolithasis. The methods used in this study are in-vitro nucleation assay, in-vitro Crystallization assay and Chorion allantois membrane (CAM) assay. To enhance the health benefits of green tea it was combined with herbs like Ocimum gratissimum, Cymbopogon citratus and Cymbopogon flexuous. The results demonstrated that The Ocimum gratissimum (tulsi), Cymbopogan cutratus and C. citratus (lemon grass) showed antilithiatic activity but they have less antilithiatic potential as compared to the standard drug cystone. The statistical data showed that there is no significant difference between C. flexuosus and O. gratissium plus green tea as compared to cystone. The antiangiogenic potential of these herbal teas could not be evaluated because of contamination in the chick embryos due to which they could not survive and the antiangiogenic potential of herbal teas could not came into conclusion.