Phytochemical screening, free radical scavenging activity, In-vitro Alpha-amylase enzyme and glucose diffusion inhibition activity of ethyl acetate and water extracts of selected medicinal plants of Nepal

Abstract

Diabetes mellitus is the metabolic disease leading to elevation of blood glucose, which may be due to the lack of enough insulin or responsible tissue become insulin resistance. The main aim of the present study was to evaluate in-vitro glucose diffusion and alpha-amylase inhibition as well as to determine free radical scavenging effects of selected plant sample. The five different plants i.e., Amomum subulatum Roxb., Choerospondias axillaris (Roxb.) B.L. Burtt, Nephrolepsis cordifolia (L). C. Presl, Musa sp. And Myrica esculenta Buch. -Ham. ex D. Don were collected from different area of Kaski district, Nepal. Myrica esculenta stem bark showed potent DPPH free radical scavenging effect in both solvent which showed IC50 value of 5.920µg/ml. Amomum subulatum, Choerospondias axillaris, Nephrolepsis cordifolia fruit and leaves and Musa species leaves in water have IC50 value of 0.81 mg/ml, 0.69mg/ml, 0.49mg/ml, 0.77mg/ml and 0.61mg/ml showing better alpha amylase inhibition. Glucose diffusion inhibition study revealed that Amomum subulatum seed, Choerospondias axillaries fruit and Musa species. Leaves showed highest GDRI% in water at concentration of 20mg/ml and 40 mg/ml. Thus, Myrica esculenta stem bark, Choerospondias axillaris fruit and Musa species leaves were found potent antioxidant and alpha amylase inhibitors.