Antioxidant and protective properties of fish meal containing different inclusion levels of Sesame indicum (Beni seed) on acetaminophen-induced oxidative stress in Clarias gariepinus

Abstract

This study analyzed the antioxidant and protective properties of fish meal containing different inclusion levels of Sesame indicum (Beni seed) on acetaminophen-induced oxidative stress in Clarias gariepinus. A total of 140 of C. gariepinus with 332.16.5±5.20g and 34.25±0.43cm as mean weight and length were grouped into 7 tanks with 20 C. gariepinus per tank. Each tank was fed separate formulated diet, namely; DT1 (commercial diet plus acetaminophen), stand as the negative control group, DT2 (commercial diet only), as a positive control, DT3 (0% S. indicum with 100% soya bean meal, DT4 (25% S. indicum with 75% soya bean meal, DT5 (50% S. indicum with 50% soya bean, DT6 (75% S. indicum with 25% soya bean and DT7 (100% S. indicum with 0% soya bean for four (4) weeks. Antioxidant properties of the prepared diets, the serum marker enzymes, and chemistry of C. gariepinus fed different diets were examined. High levels of serum marker enzymes, blood glucose, serum bilirubin, total cholesterol, and creatinine and a significant decreased in serum total protein and albumin were observed in C. gariepinus fed control diet plus acetaminophen compared to those fed the control diet (DT2) only. Inversely, low levels of serum marker enzymes, blood glucose, serum bilirubin, total cholesterol, and creatinine, and increase levels of total protein and albumin were detected in C. gariepinus fed formulated diets with varying grades of S. indicum inclusion plus acetaminophen compared to those fed commercial diet plus acetaminophen intubation. The findings of this work showed that all formulated diets with varying percentages of S. indicum inclusion are good macrocellular damage repairer in stressful C. gariepinus. However, formulated diets (DT5 and DT6) looked more promising in combating oxidative stress related disorders in C. gariepinus.