Tobacco leaf dust as natural anaesthetic to GIFT tilapia

Abstract

The present study evaluated the anaesthetic activity of tobacco (Nicotiana tabacum) leaf dust in fish. GIFT tilapia was used as an experimental animal with the total study duration of 24 hours. The fish size of 4.58±0.68 g was randomly distributed into the 20-liter plastic tub at a stocking density of 10 fishes per container. Anaesthetics were added into experimental tubs of seven different groups viz. one control (0 mg/L), followed by six treatment groups (25, 50, 75, 100, 125 and 150 mg/L) in triplicates. Results showed that an increase in the concentration of tobacco leaf dust shortened the induction time, but delayed the recovery time in fish (p